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| Yan,PM; Zhang,HF; Wang,Q; Yan,XY; Sun,Y. |
| Isozymes of peroxidase (POD), catalase (CAT), esterase (EST) and superoxide dismutase (SOD) were analyzed on transgenic maize (with external chitinase gene) and its parent by vertical polyacrylamide gel electrophoresis (PAGE). This study was made using shoots at the fourth leaf stage. Results showed that: POD and EST were detected in 6 bands. POD-2 and POD-3 were present at the bud and seedling stages. POD-1, POD-4, POD-5 and POD-6 were only present at the seedling stage. POD-6 expressed stronger in the transgenic maize with chitinase than in its parent. EST-2 was present only at the bud stage, and its expression in transgenic maize was stronger than that in its parent. EST-5 only existed at the seedling stage. EST-4 did not exist in the parent maize... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chitinase; Isozyme; Maize; Antioxidant enzyme system. |
| Ano: 2010 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200002 |
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| Santos, Carlos Henrique dos Anjos dos; Instituto Nacional de Pesquisas da Amazônia; Leitão, Marco Aurélio de Brito; Instituto Nacional de Pesquisas da Amazônia; Sousa, Carolina Fernandes Silva de; Instituto Nacional de Pesquisas da Amazônia; Santana, Givanildo Ximenes; Instituto Nacional de Pesquisas da Amazônia; Paula-Silva, Maria de Nazaré; Instituto Nacional de Pesquisa da Amazônia; Almeida-Val, Vera Maria Fonseca de; Instituto Nacional de Pesquisas da Amazônia. |
| Tambaqui (Colossoma macropomum) is among the most important fish species of the Amazon and one of the most cultivated in Brazil. In the present work we have evaluated the genetic variability of wild and captivity populations of C. macropomum. Enzymatic markers were used to estimate the genetic variability of 41 specimens from a wild group; and 30, 33 and 45 from three captivity groups, which came from Pentecostes (Ceará State), Jaboticabal (São Paulo State) and Itacoatiara (Amazonas State), respectively. Nine isoenzymic systems were used to evaluate the genetic variability of these populations. Using zimogram data we obtained the polymorphism level, allele number, allelic frequency, observed and expected heterozigosity, Wright F statistics (FIS, FST),... |
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Palavras-chave: Conservational genetics; Isozyme; Fish management; Tambaqui. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7149 |
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| Carrera,Alicia D.; Pizarro,G.; Poverene,M.; Feingold,S.; León,A.J.; Berry,S.T.. |
| Eight isozyme systems were used in this study: acid phosphatase (ACP), alcohol dehydrogenase (ADH), esterase (EST), glutamate dehydrogenase (GDH), malate dehydrogenase (MDH), phosphoglucoisomerase (PGI), 6-phosphogluconate dehydrogenase (PGD), and phosphoglucomutase (PGM). The polymorphism of these enzyme systems was studied in 25 elite inbred lines. A total of 19 loci were identified, but only eight of them were polymorphic in the germplasm tested. The polymorphic index for the eight informative markers ranged from 0.08 to 0.57, with a mean value of 0.36. Five isozyme loci were mapped in F2:3 populations with existing RFLP data. Est-1, Gdh-2 and Pgi-2 were mapped to linkage groups 3, 14 and 9, respectively. As in previous reports, an ACP locus and a PGD... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Isozyme; Polymorphisms; RFLP markers; Sunflower; Linkage map; Helianthus annuus. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100013 |
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